Histopathology service forms the core of patient care and also training of pathology residents. Wide range of specimens are received from BSMMU hospital ans outside. The usual reporting time is 3 days i.e. most of the reports are signed out by 2 p.m. on 3rd day. However, additional time required for special stain(s) and submission of more blocks.
SAMPLES SHOULD BE SENT IN 10% FORMALIN
A REQUISITION FORM WITH RELEVANT INFORMATION IS NEEDED.
FROZEN SECTION
FROZEN SECTION PROCEDURE Frozen section performed mainly for rapid microscopic analysis. The main steps in this procedure is cryosection –made by cryostat. The report is given within 15- 30 min. It is used for rapid diagnosis e.g. during surgery for intra operative evaluation of the tumour, margins, lymph nodes or other structures such as ganglion cells in Hirschsprung disease. The other uses are in staining tissue where routine processing leads to dissolution or destruction of substances need to examine (e.g. lipid demonstration, Enzyme studies-ATPase , NADPH) and for immunoflurescence studies (Renal and skin biopsy).
Frozen section tests done in 2010 at department of Pathology
IMMUNOFLUORESCENCE
Fluorescence microscopy is done routinely on renal biopsies and requested skin biopsy samples. Tests are done on pooled samples once in a week. One sample is processed for routine stain and a second for direct immunofluorescence staining. For the later fresh samples should be sent in saline soaked gauze.
Renal biopsy with DIF test at the Dept. of Pathology from 2000 to 2010
2000
2001
2002
2003
2004
2005
2006
2007
2008
2009
2010
69
214
164
165
205
223
270
388
29
38
100
PHOTOMICROGRAPHY
Photomicrography service is available for academic and research purpose. High quality (8 megapixale) digital photographs are supplied in CD or Pen Drive. Please contact Dr. Nurul Kabir, Assistant Professor, for your requirements.
CYTOLOGY
The cytology services offered by the department of Pathology are:
Exfoliative cytology
Body fluids
Cervical smear
Other.
Fine Needle Aspiration Cytology (FNAC)
Non-guided
USG and CT scan guided
IMMUNOHISTOCHEMISTRY
Uses:
1. Categorization of undifferentiated malignant tumours.
2. Determination of site of origin of metastatic tumours.
3. Detection of molecules that have prognotic or therapeutic significance.
Guideline for immunohistochemistry test:
· Specimen/Paraffin blocks received every weekdays from 8.00 am to 2.30 pm.
· Previous histopathology report and clinical information needed.
· Report delivered within two weeks (sometime longer period required to issue report in uncommon/difficult cases).
Name and price of the Immunohistochemistry markers available at present:
1. ER/PR receptors and Her –2 neu (breast cancer markers) (Tk. 5000)